Table 1.
Primer sequences used in real-time RT-PCR
| Products length (bp) |
Ta
(°C) |
Primers length (bp) | Sequences 5' 3ˊ |
Primers
name |
Genes |
|---|---|---|---|---|---|
| 162 | 54 | 25 | ACTCTGGTAAAGTGGATATTGTTGC | Sence | GAPDH |
| 21 | GGAAGATGGTGATGGGATTTC | Antisence | |||
| 169 | 54 | 18 | GCTTCAGGGTTTCATCCA | Sence | Oct3/4 |
| 18 | GGCGGCAATCATCCTCTG | Antisence | |||
| 129 | 53 | 20 | TAACAGTTCCTGCATGGGCGGC | Sence | CD44 |
| 21 | CGTGCAAATTCACCAGAAGGC | Antisence | |||
| 115 | 54 | 24 | CAACATCACAGAGGAAGTAGACTG | Sence | Sox2 |
| 19 | CCTTGGCATGAGATGCAGG | Antisence | |||
| 167 | 59 | 21 | AACTCTCCAACATCCTGAACC | Sence | Nanog |
| 22 | GTGGTAGGAAGAGTAAAGGCTG | Antisence | |||
| 171 | 59 | 21 | GAACCCACACAGGTGAGAAAC | Sence | KLF4 |
| 19 | TGTGTAAGGCGAGGTGGTC | Antisence | |||
| 70 | 58 | 18 | TCAGGGAGTCAGGGGAGG | Sence | miR-4270 |
| 19 | GAACATGTCTGCGTATCTC | Antisence | |||
| 50 | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCCCTC | RT |
Ta, annealing temperature; RT, reverse transcription