Figure 2. Sensory denervation influences the composition of tumor-infiltrating leukocytes.

Flow cytometry analyses of B16F10 tumor-infiltrating leukocytes from control C57BL/6 mice and mice after SSD. The following cells as percentages of indicated populations are shown: (A) CD45⁺ leukocytes, (B) CD45⁺CD3⁺, CD45⁺CD3⁺CD4⁺, CD45⁺CD3⁺CD8⁺, CD45⁺CD19⁺, CD45⁺CD11b⁺, CD45⁺CD11b⁺F4/80⁺ cells, (C) CD45⁺CD3⁺CD4⁺CD25⁺FoxP3⁺ T_reg, (D) CD45⁺F4/80⁺CD206⁺ macrophages, CD45⁺CD11b⁺Ly6C^hiLy6G⁻ and Ly6C^loLy6G⁺ MDSCs, (E) CD4⁺PD-1⁺, CD8⁺PD-1⁺ T cells, and (F) CD45⁺CD3⁺CD4⁺CD73⁺ cells. Data are mean±SD, two-sided student’s t-test. *P<0.05, **P<0.01, ***P<0.001; n=24–30 tumors.