Table 4.
Pancreatic and intestinal digestive enzyme specific activities on the intestine of meagre juvenile fed three diets with different incorporations of insect meal (IM).
| Control | Ins10 | Ins20 | |
|---|---|---|---|
| Specific activity | |||
| Trypsin | 4.7 ± 0.8 | 4.8 ± 1.3 | 6.8 ± 1.7 |
| Amylase | 0.9 ± 0.1 | 0.9 ± 0.1 | 1.1 ± 0.1 |
| Acid phosphatase | 1.3 ± 0.4 | 1.8 ± 0.2 | 1.5 ± 0.3 |
| Alkaline phosphatase | 115.0 ± 19.5 | 127.0 ± 14.0 | 128.6 ± 34.4 |
| Amino peptidase | 65.1 ± 2.0 | 68.6 ± 4.8 | 77.5 ± 15.1 |
| Brush border | |||
| Alkaline phosphatase | 839.4 ± 78.4 | 793.0 ± 99.9 | 763.4 ± 227.4 |
| Amino peptidase | 452.5 ± 50.8 | 445.3 ± 9.9 | 446.6 ± 111.5 |
| Total activity | |||
| Trypsin | 271.7 ± 58.2 | 257.3 ± 80.5 | 308.4 ± 26.6 |
| Amylase | 49.9 ± 7.7 | 49.3 ± 0.9 | 51.0 ± 7.2 |
| Acid phosphatase | 77.3 ± 20.4 | 104.4 ± 11.1 | 68.8 ± 22.3 |
| Alkaline phosphatase | 6689.4 ± 1000.7 | 7321.3 ± 1183.1 | 5836.5 ± 666.5 |
| Amino peptidase | 3846.5 ± 87.2a | 3943.6 ± 224.0a | 3539.6 ± 88.6b |
| Brush border | |||
| Alkaline phosphatase | 266.9 ± 30.7 | 227.9 ± 35.0 | 208.4 ± 28.4 |
| Amino peptidase | 140.9 ± 27.4 | 130.7 ± 37.2 | 119.9 ± 24.1 |
| Maturation index | |||
| Alkaline phosphatase index | 436.9 ± 92.7a | 228.7 ± 39.2b | 296.3 ± 45.5b |
| Amino peptidase index | 230.4 ± 93.7 | 133.3 ± 43.2 | 175.9 ± 19.9 |
Control had no IM, Ins10 had 10% IM incorporation in the diet, and Ins20 had 20% IM incorporation in the diet. The values are the mean and standard deviation. Specific activity is expressed by mU/mg protein, and total activity is expressed by U/segment. Maturation is the relation between the total activity of brush border enzymes and acid phosphatase. Different letters represent significant differences for p < 0.05.