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. 2023 Jul 1;9:217. doi: 10.1038/s41420-023-01531-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A, B Left: cell lysates of EBs at d7 of differentiation were analyzed by immunoblotting using indicated antibodies. Right: graphs showing quantification of immunoblots. C Left: cell lysates from undifferentiated iPSCs (day 0), or EBs at the indicated days of differentiation were analyzed by immunoblotting using the antibodies shown. Loading control: Ponceau-S staining. Right: quantification of immunoblots (n = 3). Data are represented as mean ± SEM from at least three independent experiments. The one-tailed Student’s t-test was used to assess statistical significance between genotypes (*P < 0.05; **P < 0.01; ****P < 0.0001).