Fig. 7. MAPK activation profile during WT and Gdap1-/- MN differentiation.
Left: cell lysates from undifferentiated or MN-differentiated iPSCs were analyzed by immunoblotting at the indicated timepoints. Loading control: Ponceau-S staining. Right: graphs showing quantification of MAPK phosphorylation (n = 3). Data are represented as the mean ± SEM from at least three independent experiments. The one-tailed Student’s t-test was used to assess statistical significance between genotypes (*P < 0.05; ***P < 0.001).