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. 2023 Jul 1;9:217. doi: 10.1038/s41420-023-01531-w

Fig. 7. MAPK activation profile during WT and Gdap1-/- MN differentiation.

Fig. 7

Left: cell lysates from undifferentiated or MN-differentiated iPSCs were analyzed by immunoblotting at the indicated timepoints. Loading control: Ponceau-S staining. Right: graphs showing quantification of MAPK phosphorylation (n = 3). Data are represented as the mean ± SEM from at least three independent experiments. The one-tailed Student’s t-test was used to assess statistical significance between genotypes (*P < 0.05; ***P < 0.001).