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. 2023 Jul 1;9(1):14. doi: 10.1038/s41514-023-00111-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Monocytes (THP-1) were cultured with iDC or mDC differentiation cocktail in 1 mg/mL (loose) and 3 mg/mL (dense) 3D collagen matrices either under static or RPM conditions for 3 days. iDCs and mDCs were then assessed for phenotypic changes by analysis of surface markers, cytokine secretion, and RNA sequencing. Following this differentiation, FITC-OVA was added to iDCs for 1 h under the same conditions to assess functional uptake ability. T cells were cocultured with mDCs for an additional 3 days, either under static or RPM conditions to determine the effects on mDC function in terms of activating T cells and changes in cytokine secretion. The scale bar represents 25 µm.