Table 2.
Composition of experimental diets for experiment
| Item | Phase 1 | Phase 2 | Phase 3 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CON | SPCa | ESBa | FSBLa | FSBBa | CON | SPC | ESB | FSBL | FSBB | CON | SPC | ESB | FSBL | FSBB | |
| Ingredientb, % | |||||||||||||||
| Corn, yellow | 28.79 | 27.78 | 27.96 | 27.60 | 28.13 | 40.39 | 39.48 | 39.65 | 39.24 | 39.82 | 62.58 | 61.85 | 61.92 | 61.62 | 62.02 |
| Whey permeate | 24.00 | 24.00 | 24.00 | 24.00 | 24.00 | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Cookie meal | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 10.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Soybean meal (48% CP) | 16.00 | 16.00 | 16.00 | 16.00 | 16.00 | 19.00 | 19.00 | 19.00 | 19.00 | 19.00 | 23.00 | 23.00 | 23.00 | 23.00 | 23.00 |
| Poultry meal | 10.00 | 6.67 | 6.67 | 6.67 | 6.67 | 8.00 | 2.67 | 2.67 | 2.67 | 2.67 | 4.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Fish meal (63% CP) | 4.00 | 2.67 | 2.67 | 2.67 | 2.67 | 2.00 | 0.67 | 0.67 | 0.67 | 0.67 | 1.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Blood plasma | 4.00 | 2.67 | 2.67 | 2.67 | 2.67 | 2.00 | 0.67 | 0.67 | 0.67 | 0.67 | 1.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| X-Soy200 | 0.00 | 6.50 | 0.00 | 0.00 | 0.00 | 0.00 | 7.80 | 0.00 | 0.00 | 0.00 | 0.00 | 6.00 | 0.00 | 0.00 | 0.00 |
| HP300 | 0.00 | 0.00 | 6.00 | 0.00 | 0.00 | 0.00 | 0.00 | 7.20 | 0.00 | 0.00 | 0.00 | 0.00 | 5.54 | 0.00 | 0.00 |
| Fermex200 | 0.00 | 0.00 | 0.00 | 7.09 | 0.00 | 0.00 | 0.00 | 0.00 | 8.51 | 0.00 | 0.00 | 0.00 | 0.00 | 6.54 | 0.00 |
| Soytide | 0.00 | 0.00 | 0.00 | 0.00 | 6.62 | 0.00 | 0.00 | 0.00 | 0.00 | 7.95 | 0.00 | 0.00 | 0.00 | 0.00 | 6.11 |
| Poultry fat | 1.30 | 1.50 | 1.70 | 1.00 | 1.00 | 1.30 | 1.80 | 2.10 | 1.20 | 1.30 | 1.00 | 1.30 | 1.60 | 0.90 | 1.00 |
| L-Lys HCl | 0.52 | 0.53 | 0.54 | 0.54 | 0.54 | 0.51 | 0.52 | 0.53 | 0.53 | 0.53 | 0.42 | 0.43 | 0.43 | 0.43 | 0.43 |
| L-Met | 0.25 | 0.24 | 0.25 | 0.25 | 0.23 | 0.21 | 0.20 | 0.21 | 0.21 | 0.18 | 0.14 | 0.13 | 0.14 | 0.14 | 0.12 |
| L-Thr | 0.17 | 0.11 | 0.17 | 0.17 | 0.15 | 0.15 | 0.09 | 0.16 | 0.16 | 0.13 | 0.12 | 0.07 | 0.12 | 0.12 | 0.10 |
| L-Trp | 0.02 | 0.01 | 0.01 | 0.02 | 0.01 | 0.01 | 0.00 | 0.00 | 0.02 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| L-Val | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.01 | 0.02 | 0.01 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Dicalcium phosphate | 0.00 | 0.05 | 0.05 | 0.00 | 0.00 | 0.15 | 0.65 | 0.65 | 0.60 | 0.60 | 0.54 | 0.90 | 0.90 | 0.90 | 0.90 |
| Limestone, ground | 0.30 | 0.62 | 0.66 | 0.67 | 0.66 | 0.63 | 0.80 | 0.83 | 0.85 | 0.82 | 0.80 | 0.92 | 0.95 | 0.95 | 0.92 |
| Zinc oxide | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| Salt | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 |
| Mineral premixc | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 |
| Vitamin premixd | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 | 0.03 |
| Calculated composition (as-is) | |||||||||||||||
| Dry matter, % | 91.3 | 91.4 | 91.2 | 91.0 | 91.0 | 90.7 | 90.8 | 90.6 | 90.4 | 90.4 | 89.7 | 89.8 | 89.6 | 89.4 | 89.4 |
| ME, kcal/kg | 3,454 | 3,454 | 3,449 | 3,452 | 3,449 | 3,435 | 3,437 | 3,435 | 3,436 | 3,437 | 3,373 | 3,373 | 3,374 | 3,373 | 3,376 |
| Crude protein, % | 24.6 | 24.4 | 23.8 | 23.8 | 24.1 | 22.5 | 21.9 | 21.2 | 21.2 | 21.5 | 20.9 | 20.5 | 19.9 | 19.9 | 20.1 |
| SIDe Lys, % | 1.50 | 1.50 | 1.50 | 1.50 | 1.50 | 1.35 | 1.35 | 1.35 | 1.35 | 1.35 | 1.23 | 1.23 | 1.23 | 1.23 | 1.23 |
| SID Met+Cys, % | 0.82 | 0.82 | 0.82 | 0.82 | 0.82 | 0.74 | 0.74 | 0.74 | 0.74 | 0.74 | 0.68 | 0.68 | 0.68 | 0.68 | 0.68 |
| SID Trp, % | 0.25 | 0.25 | 0.25 | 0.25 | 0.25 | 0.22 | 0.22 | 0.22 | 0.22 | 0.22 | 0.20 | 0.21 | 0.21 | 0.20 | 0.21 |
| SID Thr, % | 0.88 | 0.88 | 0.88 | 0.88 | 0.88 | 0.79 | 0.79 | 0.79 | 0.79 | 0.79 | 0.73 | 0.73 | 0.73 | 0.73 | 0.73 |
| Ca, % | 0.85 | 0.85 | 0.85 | 0.85 | 0.85 | 0.80 | 0.80 | 0.80 | 0.80 | 0.80 | 0.70 | 0.70 | 0.70 | 0.70 | 0.70 |
| STTD Pf, % | 0.50 | 0.45 | 0.45 | 0.45 | 0.45 | 0.40 | 0.40 | 0.40 | 0.40 | 0.40 | 0.33 | 0.33 | 0.33 | 0.33 | 0.33 |
| Total P, % | 0.73 | 0.67 | 0.66 | 0.65 | 0.65 | 0.63 | 0.62 | 0.62 | 0.60 | 0.61 | 0.58 | 0.57 | 0.57 | 0.56 | 0.56 |
| Glycining, mg/g | 18.0 | 18.0 | 18.0 | 29.7 | 22.4 | 21.4 | 21.4 | 21.4 | 35.4 | 26.7 | 25.9 | 25.9 | 25.9 | 36.7 | 30.0 |
| β-Conglycininh, mg/g | 20.0 | 20.0 | 20.0 | 29.8 | 21.4 | 23.8 | 23.8 | 23.8 | 35.5 | 25.4 | 28.8 | 28.8 | 28.8 | 37.8 | 30.0 |
| Analyzed composition (as-is) | |||||||||||||||
| Dry matter, % | 91.7 | 91.6 | 91.8 | 91.3 | 91.5 | 90.4 | 90.3 | 90.8 | 90.4 | 90.9 | 88.4 | 88.4 | 88.5 | 88.3 | 88.3 |
| Crude protein, % | 24.6 | 24.0 | 23.3 | 23.1 | 23.8 | 21.3 | 21.2 | 20.8 | 19.7 | 21.1 | 20.5 | 19.9 | 19.6 | 19.0 | 18.8 |
| Neutral detergent fiber, % | 8.1 | 7.9 | 7.8 | 7.6 | 7.6 | 9.1 | 8.6 | 8.6 | 8.3 | 8.2 | 9.8 | 9.6 | 9.0 | 8.8 | 9.4 |
| Acid detergent fiber, % | 3.0 | 3.2 | 3.3 | 3.0 | 3.5 | 3.5 | 3.9 | 3.7 | 3.7 | 3.7 | 4.1 | 3.9 | 4.0 | 4.0 | 4.1 |
| Ca, % | 0.77 | 0.78 | 0.86 | 0.80 | 0.81 | 0.76 | 0.74 | 0.79 | 0.77 | 0.79 | 0.68 | 0.67 | 0.73 | 0.74 | 0.62 |
| Total P, % | 0.71 | 0.66 | 0.66 | 0.65 | 0.66 | 0.62 | 0.62 | 0.62 | 0.58 | 0.60 | 0.57 | 0.58 | 0.55 | 0.53 | 0.54 |
aSPC, ESB, FSBL, and FSBB: basal diet with soy protein concentrate (X-Soy 200, CJ Selecta, Araguari, MG, Brazil), enzyme-treated soybean meal (HP 300, Hamlet Protein Inc., Findlay, OH, USA), fermented soybean meal with Lactobacillus (FerMex 200, Purina Animal Nutrition, Shoreview, SD, USA), and fermented soybean meal with Bacillus (Soytide, CJ Bio, Seoul, Korea), replacing 1/3, 2/3, 3/3 of animal protein supplements in P1, P2, P3, respectively
bThe nutritional composition of corn, soybean meal, fish meal, blood plasma, and poultry fat were from NRC [23]; the nutritional composition of poultry meal were from Rojas and Stein [24]; the nutritional composition of whey permeate, cookie meal, X-Soy200, HP300, Fermex200, and Soytide were from analyzed values (International Ingredient Corporation, Fenton, MO, USA; Darling Ingredients, Irving, TX, USA; CJ Selecta, Araguari, MG, Brazil; Hamlet Protein Inc., Findlay, OH, USA; Purina Animal Nutrition, Shoreview, SD, USA; CJ Bio, Seoul, Korea); the nutritional composition of all amino acids were from analyzed values (CJ Bio, Seoul, Korea); the nutritional composition of dicalcium phosphate, limestone, salt, mineral premix, and vitamin premix were from analyzed values (North Carolina State University Feed Mill Education Unit, Raleigh, NC, USA); the nutritional composition of zinc oxide was from analyzed values (Zinc Nacional, Monterrey, N.L., Mexico)
cThe trace mineral premix provided per kilogram of complete diet: 33 mg of Mn as manganous oxide, 110 mg of Fe as ferrous sulfate, 110 mg of Zn as zinc sulfate, 16.5 mg of Cu as copper sulfate, 0.30 mg of I as ethylenediamine dihydroiodide, and 0.30 mg of Se as sodium selenite
dThe vitamin premix provided per kilogram of complete diet: 6,614 IU of vitamin A as vitamin A acetate, 992 IU of vitamin D3, 19.8 IU of vitamin E, 2.64 mg of vitamin K as menadione sodium bisulfate, 0.03 mg of vitamin B12, 4.63 mg of riboflavin, 18.52 mg of D-pantothenic acid as calcium pantothenate, 24.96 mg of niacin, and 0.07 mg of biotin
eSID Standardized ileal digestibility
fSTTD P Standardized total tract digestible phosphorus
gThe concentration of glycinin in the diets was calculated based on the analyzed glycinin of soy protein supplements
hThe concentration of β-conglycinin in the diets was calculated based on the analyzed β-conglycinin of soy protein supplements