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. 2023 Mar 22;192(3):2507–2522. doi: 10.1093/plphys/kiad188

Figure 7.

Figure 7.

PUB13-mediated PSKR1 ubiquitination affected PSK- initiated tomato defense against B. cinerea. A, B) Effect of Lys-to-Arg mutated PSKR1 variants on plant defense to B. cinerea in N. benthamiana leaves. A) Representative images of disease symptoms on N. benthamiana leaves at 5 d postinoculation with B. cinerea (dpi). Images shown were digitally extracted and scaled for comparison. B) Quantification of B. cinerea-induced lesion area of N. benthamiana leaves at 5 dpi. Agrobacterium carrying the indicated binary vectors were transiently expressed in N. benthamiana leaves for 2 d, and then leaves were used for B. cinerea dipping inoculation. C, D) Effect of Lys-to-Arg mutated PSKR1 variants on plant defense to B. cinerea in tomato OE-PUB13 lines. C) Representative images of disease symptom on tomato leaves at 3 dpi. Images shown were digitally extracted and scaled for comparison. D) Quantification of B. cinerea-induced lesion area of indicated tomato leaves at 3 dpi. Agrobacterium carrying the indicated binary vectors were transiently expressed in tomato OE-PUB13 lines for 2 d, and then leaves were used for B. cinerea dipping inoculation. E to H) Effects of PSK on defense of OE-PUB13 and WT plants to B. cinerea infection. E) Representative leaf images for disease symptoms at 3 d postinoculation with B. cinerea (dpi). Bar = 250 μm. F) The representative chlorophyll fluorescence imaging of ΦPSII at 3 dpi. The value below each individual image indicates the degree of ΦPSII. G) Relative B. cinerea actin transcript abundance in infected tomato leaves at 1 dpi. Five-wk-old indicated tomato plants were treated with 10 μM PSK or dH2O control 12 h before B. cinerea inoculation. H) Effects of PSK application on the gene expression of B. cinerea-induced genes PAD3 and SAG12 in tomato OE-PUB13 and WT plants at 1 dpi. The transcript abundance of each gene under mock treatment in WT plants was defined as 1. Five-wk-old tomato OE-PUB13 and WT plants pretreated with 10 μM PSK or dH2O control for 1 d were sprayed with B. cinerea spore suspension. Data are presented in (B, D, F to H) as the means of three biological replicates (± SD, n = 6 in B, D; 3 in F to H), and different letters indicate significant differences (P < 0.05) according to Tukey's test.