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. 2021 Nov 29;13(8):2083–2095. doi: 10.1007/s13346-021-01095-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Quantitative apoptotic analysis in LNCaP cells treated with BL-NLC, pure CRN and CRN-NLC. A Treatment with a concentration of 25, 50 or 100 g/ml of CRN-NLC dosage for 24 h had a dose-dependent effect on early apoptosis, as evaluated by flow cytometry. B Treatment with concentrations of 25, 50 and 100 g/ml of CRN-NLC dosages for 24 h had a dose-dependent influence on late apoptosis, as measured by flow cytometry (bar charts). (*) p < 0.05, control vs. CRN-NLC (25 µg/ml), (**) p < 0.05, control vs. CRN-NLC (50 µg/ml), (***) p < 0.05, control vs. CRN-NLC (100 µg/ml) and C PE Annexin V vs. 7-AAD dot plots are used to represent dose-dependent effects. Top right: late apoptotic cells/early necrotic cells; Top left: necrotic cells; bottom right: early apoptotic cells; bottom left: live cells; and (n = 3)