FIGURE 9.

Glutamine synthetase (GS) kidney expression in WT and KO_b/c mice after 2-day MAc-challenge. (A,B) Representative low and high magnification light-microscopy images of GS immunostaining in WT (A) and KO_b/c (B) mice exposed to 2-days of MAc-challenged conditions. High magnification images of cortex and OSOM regions of interest were used for quantification of GS expression in each region. Asterisks signify tubule lumens. (C) Quantification of GS expression in the cortex and OSOM of WT and KO_b/c mice normalized such that the average in WT cortex is 100%. Comparisons between WT and KO_b/c groups assessed by Students 2-tailed unpaired t-test; *p < 0.05; ns, non-significant. N = 3M/3F per group.