TABLE 2.
Metabolic and electrolyte status of WT and KOb/c mice over 1–3 days of control or MAc conditions.
| Parameter | WT | KOb/c | p-value |
|---|---|---|---|
| Control conditions | |||
| Body weight (g) | 15.5 ± 0.2 (64) | 13.9 ± 0.3 (55) | <0.001* |
| Daily food intake (g/day) | 4.2 ± 0.1 (64) | 3.7 ± 0.1 (55) | <0.001* |
| Daily food intake (g/day/body weight) | 0.270 ± 0.004 (64) | 0.271 ± 0.004 (55) | 0.643 |
| Daily fluid intake - 0.5% sucrose (mL/day) | 6.1 ± 0.1 (64) | 5.6 ± 0.1 (55) | 0.022* |
| Daily fluid intake - 0.5% sucrose (mL/day/body weight) | 0.39 ± 0.01 (64) | 0.41 ± 0.01 (55) | 0.229 |
| Plasma [Na+], mEq/L | 145.3 ± 0.5 (27) | 144.0 ± 0.3 (24) | 0.006* |
| Plasma [Cl−], mEq/L | 109.9 ± 0.6 (26) | 106.8 ± 0.4 (24) | <0.001* |
| BUN, mg/dL | 31 ± 1 (27) | 24 ± 1 (23) | <0.001* |
| MAc conditions | |||
| Body weight (g) | 14.6 ± 0.2 (75) | 13.3 ± 0.3 (58) | 0.003* |
| Daily food intake (g/day) | 3.2 ± 0.1 (75) | 2.9 ± 0.1 (58) | <0.001* |
| Daily food intake (g/day/body weight) | 0.223 ± 0.004 (75) | 0.219 ± 0.003 (58) | 0.081 |
| Daily fluid intake - 0.28M NH4Cl + 0.5% sucrose (mL/day) | 5.1 ± 0.1 (75) | 4.9 ± 0.3 (58) | 0.083 |
| Daily fluid intake - 0.28M NH4Cl + 0.5% sucrose (mL/day/body weight) | 0.35 ± 0.01 (75) | 0.37 ± 0.01 (58) | 0.965 |
| Plasma [Na+], mEq/L | 149.1 ± 0.5 (32) | 148.7 ± 0.3 (26) | 0.657 |
| Plasma [Cl−], mEq/L | 120.7 ± 0.7 (32) | 117.5 ± 1.2 (25) | <0.001* |
| BUN, mg/dL | 40 ± 1 (32) | 35 ± 2 (26) | 0.001* |
Values represent mean ± SEM, from all experiments where animals were kept under 1, 2, or 3-days of control or metabolic acidosis (MAc) conditions. Numbers in parentheses are the numbers of animals in each group. See Table 1 for per-day sample sizes. p-values calculated by 2-way ANOVA.