Figure 6.

Creation of synthetic promoters for predictable gene expression in C. burnetii. (A) Schematic of the variable strength ProSeries synthetic promoters fused upstream of the mCherry gene. The synthetic promoters are flanked by insulation regions that help prevent unwanted gene transcription. (B) Sequence of the four different ProSeries promoters (proA, proB, proC, and proD) is shown. The only difference between each promoter lies in the -10 box. (C) The four different promoter-mCherry fusions were cloned into pJB-CAT, and the resulting vectors were transformed into C. burnetii. The level of promoter activity was examined by looking at mCherry production by immunoblot. An increasing level of mCherry production was seen across the four promoter constructs.