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. 2023 Jun 19;13:1198113. doi: 10.3389/fcimb.2023.1198113

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Copyright © 2023 Elnaggar, Ardizzone, Cerca, Toh, Łaniewski, Lillis, Herbst-Kralovetz, Quayle, Muzny and Taylor

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mock vaginal communities using E and G:P:L mix standard. The equal parts G:P:L community is meant to represent a BV-like vaginal microbiome (top), G:P:L is representative of an intermediate microbiome (middle), and the 1G:9L is meant to represent an optimal vaginal microbiome (bottom). Known copy numbers are used as input, listed on the x-axis. The output is calculated using the corresponding standard and divided by the input to generate a percentage. A 100% output/input indicates the same copy number that was input was read as output from the standard. The resulting output/input was averaged and compared to 100% using two-way ANOVA, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.