Table 1.

Length and efficiencies of standards.

Standard	UP amplicon size (bp)	SP amplicon size (bp)	UP Efficiency (%)	SP Efficiency (%)
Gardnerella spp. (G)	175	290	101.79	101.23
Prevotella spp. (P)	175	290	92.53	93.05
Fannyhessea spp. (F)	174	230	101.64	102.10
Lactobacillus spp. (L)	174	253	93.51	93.57
E. coli (E)	174	275	96.10	NA
G:P:F:L	NA	NA	96.49	NA
G:P:F	NA	NA	92.38	NA
G:P:L	NA	NA	95.30	NA
1G:9L	NA	NA	95.25	NA

UP, universal primers; SP, specific primers; bp, base pairs.

NA, not applicable.