Figure 12.

The antigenic protein shows high homology with other fungal Pho88 proteins. (A) Pho88 contains a transmembrane and the Pho88 conserved domain. In S. cerevisiae, Pho88 plays a role in the maturation of secretory proteins and is required for efficient autophagy and mitophagy. It mainly localizes in the endoplasmic reticulum. Also, its role is involved with the inorganic phosphate transport pathway. The transmembrane region is shown in shaded yellow. Protein sequence alignment was performed using the web-based tool analysis Clustal Omega available at https://www.ebi.ac.uk/Tools/msa/clustalo/. BLAST analysis tool was used to identify fungal homologs. Full protein IDs are as follows: Sc Pho88 = NP_009664.1 from Saccharomyces cerevisiae; Af Pho88 = XP_748146.1 from Aspergillus fumigatus; Tm Pho88 = XP_002151103.1 from T. marneffei strain ATCC 18224; Ss Pho88 = ERS95482.1 from Sporothrix schenckii ATCC 58251; Hc Pho88 = EGC50042.1 from Histoplasma capsulatum H88. (B) Pho88 gene expression was evaluated in T. marneffei grown in yeast, mold, or conidia. Strains were grown, RNA was prepared, and gene expression was analyzed as described in the legend of Figure 3 . The experiment was performed in three biological replicates. Error bars indicate standard deviation. Statistically significant values (* P≤ 0.05, ** P≤ 0.01) are indicated.