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Single-domain antibody PET tracers successfully image tumors engineered to express GPC3. Shown are representative PET/CT images (A) and calculated SUVs (B) of 89Zr-ssHN3 and 89Zr-nHN3 in A431 and A431-GPC3+ tumor–bearing mice (n = 3) 1 h after injection. Full ex vivo biodistribution data for mice bearing A431 and A431-GPC3+ tumors are reported in Supplemental Figures 17 and 18. ***P < 0.005.
