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. 2023 Jul 2;27(13):1918–1921. doi: 10.1111/jcmm.17777

FIGURE 8.

FIGURE 8

Knockdown of HOTAIR ameliorates renal inflammation in HUA mice. (A) The protein levels of caspase‐1, NLRP3, GSDMD‐N and GSDMD‐FL, as measured by Western blot analysis; quantification normalized to GAPDH in renal tissue from hyperuricemia mice after shRNA treatment. The protein levels of caspase‐1, NLRP decreased remarkably in hyperuricemia + shHOTAIR group; *p < 0.05 compared to the hyperuricemia + vector group; n = 6 in each group. (B, C) Immunofluorescence images showing the expression of caspase‐1, NLRP3 in renal tissue from hyperuricemia mice after shRNA treatment. Tissue immunofluorescence showed that the fluorescence intensity of glomerular caspase‐1 and NLRP3 in the shRNA‐treated group was significantly lower than that in the hyperuricemia + shHOTAIR group; *p < 0.05 compared to the control group; n = 6 in each group (scale bar, 50 μm; magnification, 400×); blue: nuclear staining (DAPI), red: caspase‐1 and NLRP3 staining. (D) The serum levels of IL‐1β and IL‐18, measured by ELISA. The serum levels of IL‐1β and IL‐18 decreased remarkably in the hyperuricemia + shHOTAIR group; *p < 0.05 compared to the in the hyperuricemia + Vector group; n = 6 in each group. (E) CD68 immunohistochemistry of the renal interstitium and glomerular mesangial area in the Hyperuricemia + vector group and hyperuricemia + shHOTAIR group. Immunohistochemistry of CD68 in renal tissue from hyperuricemia mice after shRNA treatment. Compared with that in the hyperuricemia + Vector group, the downregulation of HOTAIR by shHOTAIR significantly reduced the infiltration of positive CD68 macrophages in the renal interstitium and glomerular Mesangial area in hyperuricemia + shHOTAIR group; *p < 0.05 compared to hyperuricemia + vector group; n = 6 in each group(scale bar, 100 μm; magnification, 400×).