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. 2023 May 29;27(13):1836–1858. doi: 10.1111/jcmm.17782

FIGURE 2.

FIGURE 2

Characteristics of bone marrow‐derived mesenchymal stem cells (BMSCs) under normoxic or hypoxic conditions. (A) Representative images of passages 0 (P0) and 3 (P3). (B) Morphological appearance of hypoxic preconditioned BMSCs (HP‐BMSCs) at P3. Scale bar = 100 μm. (C) Flow cytometric results indicated that BMSCs at P3 were positive for CD29 and CD90 and negative for CD11b and CD 45. (D) Cell Counting Kit‐8 (CCK‐8) analysis revealed hypoxic conditions for 48 h in culture did not promote BMSCs proliferation compared with the normoxic cultured cells (21% O2, n = 6). (E, F) Annexin V/propidium iodide (PI) showed hypoxia for 48 h and did not induce apoptosis of BMSCs (n = 5). (G, H) Transwell assay demonstrated hypoxic preconditioning increased the BMSCs migration compared with the normal‐cultured BMSCs (N‐BMSCs, n = 4). Scale bar = 100 μm. All data are presented as means ± SD. *p < 0.05 vs. the N‐BMSCs group. Ns p > 0.05 vs. the N‐BMSCs group.