Table 1.
Crystallographic and refinement statistics of two RNA duplexes, M2 and M3, presented in this study
| Data collection statistics | M2 duplex | M3 duplex |
|---|---|---|
| Sequence | r(UUCUGCUGCUGAA/UUCUGCAGCUGAA) | r(UUCUGCUGCUGAA)2 |
| Beamline | NSRRC, BL15A1 | NSRRC, TPS 05A |
| Detector type | RAYONIX MX300HE | RAYONIX MX300-HS |
| Wavelength [Å] | 1.0000 | 1.0000 |
| Data collection temperature [K] | 100 | 100 |
| Space group | P32 | P3221 |
| Cell dimensions | ||
| a, b, c [Å] | 49.22, 49.22, 35.80 | 49.26, 46.26, 36.14 |
| α, β, γ [°] | 90, 90, 120 | 90, 90, 120 |
| Resolution range [Å]a | 30.00–1.58 (1.58–1.64) | 30.00–1.87 (1.87–1.94) |
| Total reflections | 126,315 | 44,478 |
| Unique reflections | 12,483 | 4355 |
| Completeness [%]a | 94.0 (100) | 99.4 (100) |
| Mean I/σ [I]a | 30.86 (7.01) | 30.85 (7.20) |
| R-merge [%]a | 0.049 (0.398) | 0.059 (0.383) |
| Redundancya | 10.8 | 10.1 |
| Refinement statistics | ||
| R-work/R-free | 0.23/0.25 | 0.21/0.22 |
| No. of nonsolvent atoms | 550 | 274 |
| No. of solvent atoms | 113 | 58 |
| Average B-factor [Å2] | 28.2 | 30.0 |
| R.m.s.d. bond lengths [Å] | 0.004 | 0.004 |
| R.m.s.d. bond angles [°] | 0.89 | 0.85 |
| PDB code | 7Y2P | 7Y2B |
a
Outer shell statistics are shown in parenthesis.