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. Author manuscript; available in PMC: 2023 Jul 3.
Published in final edited form as: Nature. 2022 Oct 19;610(7933):775–782. doi: 10.1038/s41586-022-05333-5

Extended Data Fig. 1 ∣. Examination of bifunctional degraders of BRD4 with candidate degrons for CRBN.

Extended Data Fig. 1 ∣

(a) Representative mechanisms of the formation of C-terminal cyclic aspartimide (cN) or glutarimide (cQ) and N-terminal pyroglutamate (pE) in vivo. (b) Structures of JQ1-uracil, JQ1-PEG-uracil, and JQ1-uridine. (c) Western blot of BRD4 after treatment with JQ1-uracil, JQ1-PEG-uracil, JQ1-uridine in HEK293T cells for 24 h. (d) Structures of JQ1-cQ, JQ1-cN, and JQ1-FpE. (e) Western blot of BRD4 after treatment with JQ1-cQ, JQ1-cN, JQ1-FpE in HEK293T cells for 4 h. All western blot data are representative of at least 2 independent replicates. For uncropped western blot images, see Supplementary Fig. 8.