Fig. 4.

Global SHAP values for the $16\times 23$ CNN classification model. Negative global SHAP values (red) indicate an average predicted decrease in guide activity for the respective feature. Mismatch channels (middle three heatmaps) can be represented by the (off-)target and on-target protospacer nucleotides (left vertical axis) as well as the physical base pair interfaces (right vertical axis), such that A_mismT describes all configurations in which an adenine on the target strand faces an adenine on the sgRNA. The bottom heatmap visualises the influence of our chosen set of nucleosomal organisation features on cleavage activity. A bar representation of this can be found in Figure S5. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)