Table 1.
Plasmids used in this study
| Plasmid | Description | Reference |
|---|---|---|
| pOZ14 | A 3.1 kb pBluescript II derivative bearing a 149 bp minimal tma-oriC | (40) |
| pOZ14_Trio-A1 | A pOZ14 derivative with modified DUE | This study |
| pOZ14_Trio-A2 | A pOZ14 derivative with modified DUE | This study |
| pOZ14_U | A pOZ14 derivative with modified DUE | This study |
| pOZ14_B | A pOZ14 derivative with modified DUE | This study |
| pOZ14_Trio | A pOZ14 derivative with modified DUE | This study |
| pOZ14_U&Trio | A pOZ14 derivative with modified DUE | This study |
| pOZsub1 | A pOZ14 derivative in which the sequence of tmaDnaA box 1 is randomized | (9) |
| pOZsub2 | A pOZ14 derivative in which the sequence of tmaDnaA box 2 is randomized | (9) |
| pOZsub3 | A pOZ14 derivative in which the sequence of tmaDnaA box 3 is randomized | (9) |
| pOZsub4 | A pOZ14 derivative in which the sequence of tmaDnaA box 4 is randomized | (9) |
| pOZsub5 | A pOZ14 derivative in which the sequence of tmaDnaA box 5 is randomized | (9) |
| pOZ14_a+5 | A pOZ14 derivative with a 5 bp insert between tmaDnaA boxes 1 and 2 | This study |
| pOZ14_a+10 | A pOZ14 derivative with a 10 bp insert between tmaDnaA boxes 1 and 2 | This study |
| pOZ14_b+5 | A pOZ14 derivative with a 5 bp insert between tmaDnaA boxes 1 and 2 | This study |
| pOZ14_b+10 | A pOZ14 derivative with a 10 bp insert between tmaDnaA boxes 1 and 2 | This study |
| pOZ14_R2 | A pOZ14 derivative in which the sequence of tmaDnaA box 2 is reversed | This study |
| pTHMA-1 | A plasmid for purification of wildtype tmaDnaA | (40) |
| pTMA R251A | A pTHMA-1 derivative carrying the tmaDnaA R251A allele | This study |