Table 2.

Kinetic parameters for the hydrolysis of various substrates by FjDexUL GHs

Enzyme	Substrate	Km (mM)	kcat (s−1)	kcat/Km (s−1 mM−1)
FjGH66	Dextran 40,000	0.165 ± 0.014a (6.62 ± 0.52 mg/ml)	49.1 ± 1.81	3.07 × 102 (7.42 s−1 mg−1 ml)
	Dextran 200,000	(2.19 ± 0.27) × 10−2a (4.38 ± 0.54 mg/ml)	29.7 ± 1.14	1.36 × 103 (6.78 s−1 mg−1 ml)
FjDex31A	Dextran 40,000	(4.97 ± 0.70) × 10−2a (1.99 ± 0.28 mg/ml)	3.79 ± 0.36	76.3 (1.90 s−1 mg−1 ml)
	Dextran 200,000	(1.59 ± 0.13) × 10−2a (3.18 ± 0.26 mg/ml)	3.57 ± 0.09	2.24 × 102 (0.841 s−1 mg−1 ml)
FjGH97A	Isomaltose	2.47 ± 0.34	56.9 ± 2.8	22.9
	Isomaltotriose	0.692 ± 0.072	45.5 ± 1.2	65.8
	Isomaltotetraose	0.794 ± 0.196	55.8 ± 3.7	70.3
	Kojibiose	3.46 ± 1.86	22.9 ± 5.1	6.61
	Nigerose	2.79 ± 0.71	46.5 ± 4.3	16.6
	Maltose	1.06 ± 0.21	3.00 ± 0.15	2.81

^aCalculated from average molecular weight of dextran.