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. 2023 May 4;11(9):315. doi: 10.21037/atm-22-3670

Figure 2.

Figure 2

Blocking anti CD40 and anti uPAR antibodies rescue the actin depolarization in podocytes induced by sera from FSGS patients with recurrence of FSGS after renal transplant (rFSGS). (A) Representative images displaying stress fibers in cultured human podocytes stained with rhodamine conjugated phalloidin (green) after treatment with pre-transplant sera from three rFSGS patients. Podocyte were cultured in the presence of sera (4% final concentration) with or without pre-treatment with anti uPAR or anti CD40 blocking antibody. (B) Quantification of stress fiber positive podocytes (minimum 100 cells per condition were counted). Treatment with sera from rFSGS patients caused significant depolarization of stress fibers as determined by number of stress fiber positive cells (30%, 59% and 49% reduction with respected to untreated podocytes respectively). Pre-treatment of podocytes with novel blocking anti CD40 antibody or anti uPAR antibody (1 µg/mL) before addition of patient sera rescued stress fibers. FSGS, focal segmental glomerulosclerosis; rFSGS, recurrence of FSGS.