Figure 3.

Skin wound healing is improved in post-partum SAD mice with delayed cutaneous healing. (A) Representative images of wounds at days 0, 3, 5 and 7, and planimetry of wound area at days 5 and 7 relative to the original wound area. (B) Representative images of wounds labeled with anti-K14 antibody at day 7. The size of the neo-epidermis relative to the total wound area is provided. (C) Representative images and quantification of Ki67⁺ cells in the wound bed at day 7. (D) Representative images of CD31⁺ cells and quantification of vessel area in the wound bed at day 7. Scale bars represent 1000 mm (B) or 50 mm (C, D). In (B-D), nuclei were counterstained with DAPI. In (A-D), one 8-mm excisional wound was performed in virgin mice (n=4) or post-partum SAD mice (n=5). Data are presented as means with standard deviations and individual values. Statistical analyses were performed with two-tailed t tests with the Welch correction whenever required (A, C) or Mann-Whitney test (B, D). ns: not statistically significant; *P<0.05; ***P<0.0005.