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. 2023 Jun 13;15(25):29752–29766. doi: 10.1021/acsami.3c01481

Figure 5.

Figure 5

Substrate topographical cues regulate global histone modification. The cells were either cultured in GM for 4 days (GM (4d)) or with additional 3 days of culture in MM (GM (4d) + MM (3d)). (A) Representative H3K27me3 and H3K9ac immunofluorescence staining images of hBMSCs on different substrates (scale bar = 10 μm). (B,C) Quantification of H3K27me3/H3 and H3K9ac/H3 ratio of MFI using flow cytometry. The MFI was calculated with Flowjo software (n ≥ 4; * p < 0.05). (D,E) Quantification of globe H3K27me3 and H3K9ac in hBMSCs on hPC under different inhibiting conditions (n = 5; * p < 0.05).