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Substrate topographical cues influence the histone modification on the promoters of differentiation genes. The cells were either cultured in GM for 4 days (GM (4d)) or with additional 3 days of culture in MM (GM (4d) + MM (3d)). ChIP-PCR was performed to examine the H3K27me3 and H3K9ac on the promoters of osteogenesis and adipogenesis genes (n = 3; * p < 0.05).
