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Mechanical force mediated hBMSC differentiation toward osteolineage. The cells were first cultured in GM for 4 days to reach a confluence of 80%; then the medium was changed to MM for 14 additional days of culture. The cells were treated with inhibitors or siRNAs, and their differentiation was examined via quantification of OCN (A) and FABP-4 (B) (n = 4; * p < 0.05) and staining of calcium deposits (C) and lipid droplets (D) (Scale bar = 200 μm).
