REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
FITC anti-mouse CD45.2	Thermo Fisher Scientific	Cat# 11-0454-82; RRID: AB_465061
Brilliant Violet™ 421 anti-mouse CD19	Thermo Fisher Scientific	Cat# 404-0193-82; RRID: AB_1659676
Brilliant Violet™ 421 anti-mouse CD3ε	Thermo Fisher Scientific	Cat# 404-0031-82; RRID: AB_2925483
eFluor™ 450 anti-mouse CD5	Thermo Fisher Scientific	Cat# 48-0051-82; RRID: AB_1603250
eFluor™ 450 anti-mouse Ly-6G/Ly6C	Thermo Fisher Scientific	Cat# 48-5931-82; RRID: AB_1548788
NKp46 biotin	Thermo Fisher Scientific	Cat# 13-3351-82; RRID: AB_2572784
APC-eFluor™ 780 anti-mouse NK1.1	Thermo Fisher Scientific	Cat# 47-5941-82; RRID: AB_2735070
PE anti-mouse CD49a	BioLegend	Cat# 142604; RRID: AB_10945158
APC anti-mouse IL-21R	BioLegend	Cat# 131910; RRID: AB_2124133
APC anti-mouse CD218a (IL-18Rα)	BioLegend	Cat# 157906; RRID: AB_2738885
PE/Cyanine7 anti-mouse CD49b	BioLegend	Cat# 103518; RRID: AB_2566103
FITC anti-mouse IFN-γ	BioLegend	Cat# 505806; RRID: AB_315400
APC anti-mouse TNF-α	BioLegend	Cat# 506308; RRID: AB_315429
Brilliant Violet 711™ anti-mouse CD186 (CXCR6)	BioLegend	Cat# 151111; RRID: AB_2721558
TruStain FcX™ (anti-mouse CD16/32)	BioLegend	Cat# 101302; RRID: AB_312801
Chemicals, peptides, and recombinant proteins
Collagenase type Ⅳ	Sigma-Aldrich	Cat# C4-22-1G
DNase I recombinant, RNase-free	Roche	Cat# 4716728001
Brefeldin A	Thermo Fisher Scientific	Cat# 00-4506-51
Fixable Viability Dye eFluor™ 506	Thermo Fisher Scientific	Cat# 65-0866-14
Brilliant Violet 785™ Streptavidin	BioLegend	Cat# 405249
Recombinant Mouse IL-21 (carrier-free)	BioLegend	Cat# 574506
Recombinant Mouse IL-18	Sinobiological	Cat# 50073-MNCE
Critical commercial assays
TruePrep DNA Library Prep Kit V2 for Illumina	Vazyme	Cat# 502
SMART-Seq® HT Kit	Takara	Cat# 634437
NEBNext® Ultra™ DNA Library Prep Kit for Illumina®	New England Biolabs	Cat# E7370L
Deposited data
Data files for RNA and ATAC sequencing	This paper	GSE223677
MCMV infection dataset	Quatrini et al.37	SRP148791
NK ATAC sequencing dataset	Nixon et al.25	SRP359796
Single cell NK dataset	Lopes et al., 202231	GSE189807
scRNA-seq of human tonsil NK	Björklund et al.33	GSE70580
scRNA-seq of human NK in different tissues	Mazzurana L et al.,32	GSE150050
Software and algorithms
Flowjo v10	BD Biosciences	N/A
GraphPad Prism v9	GraphPad Software	N/A
Illustrator	Adobe	N/A
DESeq2		https://bioconductor.org/packages/release/bioc/html/DESeq2.html
clusterProfiler		https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html.org/packages/release/bioc/html/clusterProfiler.html
ssGSEA		https://www.bioconductor.org/packages/release/bioc/vignettes/GSVA/inst/doc/GSVA.html
Cytoscape		https://cytoscape.org
Trimmomatic		https://www.usadellab.org/cms/?page=trimmomatic
PICARD		https://broadinstitute.github.io/picard/
MACS		https://genomebiology.biomedcentral.com/articles/10.1186/gb-2008-9-9-r137
HOMER		http://homer.ucsd.edu/homer/
BEDtool		https://bedtools.readthedocs.io/en/latest/index.html
Seurat		https://github.com/satijalab/seurat
HISAT2		http://daehwankimlab.github.io/hisat2/
bowtie2		https://bowtie-bio.sourceforge.net/bowtie2/index.shtml