Figure 7.

Targeting the SHH pathway in cortical neurons did not improve 4H associated GABAergic phenotypes. Control and 4H neurons were treated with vehicle (DMSO) or 100 nM SAG from Day 18 to Day 56. After SAG treatment, the dendritic length per cell (A) and number of dendritic segments per cell (B), both based on MAP2 staining, were significantly decreased in control but not in 4H neurons. No significant changes in the number of synapses per dendritic length (C) or the percentage of GABAergic synapses (D) were observed. Px = pixels, *P < 0.05. Open circles represent average per individual patient/control, filled circles represent the mean per genotype. Error bars represent SEM.