(A) Mechanical pain thresholds before and after CFA in mice treated with anti-Ly6G antibody or its isotype control (vehicle). Symbols represent means ± SEM hind paw withdrawal threshold (g). (B) Percent allodynia on day 6 after drug; see Materials and Methods for calculation details. (C) Days to return to baseline; see Materials and Methods. Mice not returning to baseline on day 120 were assigned a value of 140. (D) Mechanical pain thresholds before and after CFA in mice treated with DEXA (or saline), plus a hind paw injection of vehicle, isolated neutrophils, S100A8, or S100A9 on days 3 and 5 after DEXA. Symbols as in (A). (E) Percent allodynia on day 6 after DEXA. *P < 0.05, **P < 0.01, and ***P < 0.001, compared to the corresponding vehicle/no DEXA group.