Table 1.

Effect of different purification methods on concentration, quality, and contamination of RNA extracted from distal colon tissues in young pigs¹

In vivo Group	Purification method			SEM	P-value
	No purification	LiCl	Spin column
Control
N	8	8	8
Concentration, µg/µL	1,934	1,800	1,646	88.3	0.09
260/280 ratio2	1.95a	2.04b	2.04b	0.005	<0.001
260/230 ratio3	1.91a	2.18b	2.21c	0.029	<0.001
RQN4	8.35a	5.50b	8.01a	0.659	0.011
DSS-1
N	7	7	7
Concentration, µg/µL	1,542	1,107	1,341	224.9	0.41
260/280 ratio2	1.92a	2.00b	2.05c	0.013	<0.001
260/230 ratio3	1.99	2.15	2.17	0.026	0.08
RQN4	7.41	5.57	7.40	0.572	0.05
DSS-2
n	8	8	8
Concentration, µg/µL	1,821a	1,366b	1,593a,b	175.5	0.048
260/280 ratio2	1.95a	2.03b	2.05b	0.006	<0.001
260/230 ratio3	1.91	2.18	2.21	0.029	<0.001
RQN4	8.16	6.54	7.75	0.515	0.09

¹All samples (N = 7 to 8) were snap frozen in liquid nitrogen, then stored at −80 °C pending analysis.

²The 260/280 ratio indicates the purity of RNA at wavelengths of 260 and 280 nm.

³The 260/230 ratio indicates the absence of RNA contaminants at wavelengths of 260 and 230 nm.

⁴The RQN indicates the integrity of RNA on a scale from 1 to 10, with 1 being completely degraded and 10 being completely intact RNA.

^a-cMeans lacking a common superscript letter within a row differ (P < 0.05).