Table 2.

Frequency of detectable amplification in gene expression across three purification methods in young pigs without or with DSS administration¹

In vivo group	Purification method			P-value
	No purification	LiCl	Spin column
Control2
RPL-19	100.0	100.0	100.0	1.00
GAPDH	100.0	100.0	100.0	1.00
β-actin	100.0	100.0	100.0	1.00
IFN-γ	100.0	100.0	100.0	1.00
IL-63	–	100.0	100.0	1.00
IL-123	–	100.0	100.0	1.00
IL-10	100.0	100.0	100.0	1.00
TNF-α3	–	100.0	100.0	1.00
DSS-1
RPL-19	0.00	14.3	42.9	0.041
GAPDH	28.6	14.3	57.1	0.044
β-actin	28.6	14.3	57.1	0.044
IFN-γ	100.0	14.3	42.9	0.041
IL-63	–	14.3	57.1	0.012
IL-123	–	14.3	57.1	0.012
IL-10	28.6	14.3	57.1	0.044
TNF-α3	–	0.0	57.1	0.006
DSS-2
RPL-19	0.0	0.0	37.5	0.028
GAPDH	0.0	0.0	50.0	0.007
β-actin	0.0	0.0	50.0	0.007
IFN-γ	100.0	0.0	37.5	0.028
IL-63	–	0.0	37.5	0.028
IL-123	–	0.0	25.0	0.10
IL-10	0.0	0.0	50.0	0.007
TNF-α3	–	0.0	50.0	0.007

¹Values presented as the percentage of pigs within each method that produced detectable Ct values during gene expression analysis. All samples (N = 7 to 8) were snap frozen in liquid nitrogen, then stored at −80 °C until analysis was ran. Abbreviations: GAPDH, glyceraldenyde-3-phosphate dehydrogenaseRPL19, ribosomal protein L19.

²Statistical analysis did not produce a P-value as all samples had detectable Ct values, therefore a P-value of 1 is shown.

³Gene expression was not analyzed for the no purification treatment.