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Anti-hookworm IgY characterization. A: Kinetics of IgY antibodies production. * indicate immunizations. Electrophoretical profiles in SDS-PAGE 12%. B: 1 – molecular weight, 2 – A. ceylanicum saline extract, 3 – IgY antibodies after HPLC fractionation. Immunoblotting to evaluate antigenic recognition of IgY antibodies from 4 – hens immunized with A. ceylanicum s.e. and 5 – hens immunized with PBS.
