Fig. 3.

Immunoproteomic analysis of somatic antigens from female H. polygyrus L4 from mice with colitis or controls. 2D silver stained gels of female H. polygyrus from mice with colitis (A) and female L4 from control mice (B). IEF was performed with molecules extracted from L4 with an IPG strip with pH range of 3–10. SDS-PAGE was performed on 12% gel. Western blot analyses of proteins of female H. polygyrus from mice with colitis (C, D) separated in 2D electrophoresis were transferred to PVDF membrane. The blot was probed with mouse with colitis serum (1:100), followed by HRP-conjugated anti-mouse IgG₁ (C) or anti-mouse IgA (D) and visualized by enhanced chemiluminescence. Spots detected by antibodies are indicated by arrows. The numbers refer to the table and western blot results.