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. 2020 Jul 30;147(13):1480–1487. doi: 10.1017/S0031182020001201

Table 1.

Immunogenic protein spots identified by mass spectrometry

G SN MR Description Species T MS pI NM SC% MS emPAI Ab
CM 1 VDO88828.1 Intermediate filament tail domain protein H. polygyrus 66 598 5.77 5 10 360 0.37 IgG1, IgA
CM 2 VDO62073.1 Intermediate filament protein ifb-1 H. polygyrus 55 543 5.45 6 11 349 0.35 IgG1, IgA
CM 3 VDO88828.1 Intermediate filament tail domain protein H. polygyrus 66 598 5.77 10 18 611 0.56 IgG1, IgA
CM 4 VDO88828.1 Intermediate filament tail domain protein H. polygyrus 66 598 5.77 11 19 248 0.66 IgG1
CM 5 VDP30464.1 Aldehyde dehydrogenase family protein H. polygyrus 38 886 5.44 3 7 186 0.08 IgG1, IgA
CM 6 VDP11345.1 Protein disulphide-isomerase domain protein H. polygyrus 49 091 8.53 2 6 136 0.09 IgG1, IgA
CM 7 VDO88828.1 Intermediate filament tail domain protein H. polygyrus 66 598 5.77 2 3 146 0.13 IgG1, IgA
CM 8 VDO88828.1 Intermediate filament tail domain protein H. polygyrus 66 598 5.77 17 26 1010 1.14 IgG1, IgA
CM 9 VDO62073.1 Intermediate filament protein ifb-1 H. polygyrus 55 543 5.45 5 12 328 0.26 IgG1, IgA
CM 10 VDO88828.1 Intermediate filament tail domain protein H. polygyrus 66 598 5.77 2 3 141 0.13 IgG1, IgA
CM 11 VDO59867.1 Glyceraldehyde-3-phosphate dehydrogenase, type I H. polygyrus 36 768 7.72 2 6 118 0.12 IgG1, IgA
CM 12 VDO99597.1 Actin H. polygyrus 36 852 5.17 3 12 116 0.37 IgG1
CF 13 VDO95305.1 Achain A, galectin H. polygyrus 31 673 6.07 1 4 85 0.28 IgG1, IgA
CF 14 VDP05504.1 Galactoside-binding lectin H. polygyrus 30 181 6.01 1 4 88 0.15 IgG1, IgA
CF 15 VDP45435.1 Thioredoxin domain-containing protein H. polygyrus 22 010 6.20 38 88 3320 1629.83 IgG1, IgA
CF 16 PIO59125.1 Actin Teladorsagia circumcincta 42 128 5.30 60 70 8157 992.92 IgG1, IgA
CF 17 PIO59125.1 Actin T. circumcincta 42 128 5.30 64 76 8034 1941.55 IgG1, IgA
CF 18 PIO59125.1 Actin T. circumcincta 42 128 5.30 54 69 7126 544.40 IgG1, IgA
CF 19 PIO59125.1 Actin T. circumcincta 42 128 5.30 45 61 5587 146.15 IgG1, IgA
CF 20 VDO71438.1 EF hand calcium-binding protein H. polygyrus 31 657 4.52 25 63 2282 34.81 IgG1, IgA
CF 21 VDP05504.1 Galactoside-binding lectin H. polygyrus 30 181 6.01 31 69 1929 62.88 IgG1
CF 22 VDP32743.1 Succinate dehydrogenase, flavoprotein subunit H. polygyrus 71 457 7.91 1 1 77 0.06 IgG1, IgA
M 23 PIO59125.1 Actin T. circumcintra 42 128 5.30 32 58 2779 43.41 IgG1, IgA
M 24 PIO59125.1 Actin T. circumcincta 42 128 5.30 30 76 2635 28.99 IgG1, IgA
M 25 PIO59125.1 Actin T. circumcincta 42 128 5.30 31 54 2870 35.37 IgG1, IgA
M 26 VDP32743.1 Succinate dehydrogenase, flavoprotein subunit H. polygyrus 71 457 7.91 1 1 88 0.06 IgG1, IgA
M 27 RCN53063.1 Tubulin binding cofactor A Ancylostoma caninum 13 066 5.46 1 7 64 0.37 IgA
M 28 VDP32743.1 Succinate dehydrogenase, flavoprotein subunit H. polygyrus 71 457 7.91 9 9 389 0.19 IgA
M 29 VDP43470.1 Transketolase, thiamine diphosphate binding domain protein H. polygyrus 64 326 6.69 4 5 244 0.30 IgA
M 30 VDP43470.1 Transketolase, thiamine diphosphate binding domain protein H. polygyrus 64 326 6.69 4 5 246 0.14 IgG1
M 31 VDP43470.1 Transketolase, thiamine diphosphate binding domain protein H. polygyrus 64 326 6.69 4 6 213 – IgG1
M 32 VDO76340.1 ATP synthase F1, alpha subunit H. polygyrus 55 323 8.19 4 6 213 – IgG1
M 33 KJH52568.1 Actin Dictyocaulus viviparus 40 505 5.35 3 5 183 0.23 IgG1
M 34 KIH47941.1 KETtiN (Drosophila actin-binding) homologue Ancylostoma duodenale 13 568 4.95 1 8 60 0.36 IgG1, IgA
M 35 VDO67369.1 Protein disulphide-isomerase domain protein H. polygyrus 60 016 4.99 29 46 1535 7.79 IgA
F 36 VDP05504.1 Galactoside-binding lectin H. polygyrus 30 181 6.01 1 4 88 0.15 IgG1
F 37 VDO99596.1 Actin H. polygyrus 22 979 5.44 2 12 182 0.28 IgG1, IgA
F 38 VDO67369.1 Protein disulphide-isomerase domain protein H. polygyrus 60 016 4.99 17 25 1236 2.07 IgG1, IgA
F 39 VDO67369.1 Protein disulphide-isomerase domain protein H. polygyrus 60 016 4.99 5 10 314 0.32 IgG1
F 40 KIH47941.1 KETtiN (Drosophila actin-binding) homologue A. duodenale 13 568 4.95 1 8 64 0.36 IgG1, IgA
F 41 VDO94064.1 Putative chaperone protein DnaK H. polygyrus 73 591 5.12 16 22 1118 1.11 IgG1, IgA
F 42 VDO81416.1 Putative chaperone protein DnaK H. polygyrus 70 350 5.36 2 4 101 – IgG1, IgA

SN, spot number; MR, MASCOT results (NCBI); T MS, theoretical monoisotopic mass (Da); pI, calculated isoelectronic point; MP, number of matched peptides; SC%, sequence coverage range (%); MS, MASCOT score; Ab, antibody class.

CM, HP Col Male (antigen of male L4 from DSS-treated mice); CF, HP Col Female (antigen of female L4 from DSS-treated mice); M, HP Male (antigen of male L4 H. polygyrus from control mice); F, HP Female (antigen of female L4 from control mice).