Table 1.
Taxa | Band size | Target gene | Specific primers | Primer sequence (5′-3′) | Reference |
---|---|---|---|---|---|
T. congolense savannah | 700 | ITS1 | ITS1 forward ITS2 reverse |
CCGGAAGTTCACCGATATTG TTGCTGCGTTCTTCAACGAA |
Njiru et al. (2005) |
T. congolense forest | 710 | ||||
T. congolense kilifi | 620 | ||||
T. simiae Tsavo | 370 | ||||
T. simiae | 400 | ||||
Trypanozoon | 480 | ||||
T. vivax | 250 | ||||
T. godfreyi | 300 | ||||
T. theileri | – | ||||
T. evansi | 488 | RoTat1.2 VSG | RoTat 1.2 forward RoTat 1.2 reverse |
GCCACCACGGCGAAAGAC TAATCAGTGTGGTGTGC |
Urakawa et al. (2001) |
T. simiae | 437 | Sat-DNA | TSM1 forward TSM2 reverse |
CCGGTCAAAAACGCATT AGTCGCCCGGAGTCGAT |
Masiga et al. (1992) |
T. brucei | 173 | Sat-DNA | TBR1 forward TBR2 reverse |
CGAATGAATATTAAACAATGCGCAGT AGAACCATTTATTAGCTTTGTTGC |
Moser et al. (1989) |
T. b. rhodesiense | 284 | SRA | SRA forward SRA reverse |
ATAGTGACAAGATGCGTACTCAACGC AATGTGTTCGAGTACTTCGGTCACGCT |
Radwanska et al. (2002) |