Skip to main content
. 2020 Sep 21;147(14):1786–1791. doi: 10.1017/S003118202000178X

Table 1.

PCR primers used in the present study

Taxa Band size Target gene Specific primers Primer sequence (5′-3′) Reference
T. congolense savannah 700 ITS1 ITS1 forward
ITS2 reverse
CCGGAAGTTCACCGATATTG
TTGCTGCGTTCTTCAACGAA
Njiru et al. (2005)
T. congolense forest 710
T. congolense kilifi 620
T. simiae Tsavo 370
T. simiae 400
Trypanozoon 480
T. vivax 250
T. godfreyi 300
T. theileri
T. evansi 488 RoTat1.2 VSG RoTat 1.2 forward
RoTat 1.2 reverse
GCCACCACGGCGAAAGAC
TAATCAGTGTGGTGTGC
Urakawa et al. (2001)
T. simiae 437 Sat-DNA TSM1 forward
TSM2 reverse
CCGGTCAAAAACGCATT
AGTCGCCCGGAGTCGAT
Masiga et al. (1992)
T. brucei 173 Sat-DNA TBR1 forward
TBR2 reverse
CGAATGAATATTAAACAATGCGCAGT
AGAACCATTTATTAGCTTTGTTGC
Moser et al. (1989)
T. b. rhodesiense 284 SRA SRA forward
SRA reverse
ATAGTGACAAGATGCGTACTCAACGC
AATGTGTTCGAGTACTTCGGTCACGCT
Radwanska et al. (2002)