Table 2.
Vibility testing in vivo (Meriones unguiculatus) of E. multilocularis metacestode material following long-term (33–35 years) cryopreservation
| Isolate | Date of cryopreservation | Experiment no. | Tissue type | Cryoprotectant | Animals | ||
|---|---|---|---|---|---|---|---|
| Infected | Positive for metacestodes | With abortive lesions | |||||
| A/1 | 19-09-84 | 1 | STF | 5% DMSO | 3 | 0 | 0 |
| 19-09-84 | 2 | STF | 10% glycerol | 3 | 0 | 1 | |
| 22-01-85 | 3 | TB | 5% DMSO | 3 | 0 | 2 | |
| 06-03-86 | 4 | TB | 10% glycerol | 3 | 0 | 3 | |
| CDN/1 | 27-09-84 | 5 | STF | 5% DMSO | 3 | 2 | 1 |
| 27-09-84 | 6 | STF | 10% glycerol | 3 | 0 | 2 | |
| 22-01-85 | 7 | TB | 5% DMSO | 3 | 1 | 1 | |
| 24-01-86 | 8 | TB | 10% glycerol | 3 | 0 | 0 | |
| CH/11 | 11-09-84 | 9 | STF | 5% DMSO | 3 | 1 | 2 |
| 13-09-84 | 10 | STF | 10% glycerol | 3 | 2 | 0 | |
| 20-03-85 | 11 | TB | 5% DMSO | 3 | 2 | 0 | |
| 01-07-85 | 12 | TB | 10% glycerol | 3 | 0 | 1 | |
| Altogether: | 36 | 8 | 13 | ||||
A, Alaska; CDN, Canada; CH, Switzerland; STF, small tissue fragments; TB, tissue blocks.