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. 2023 Jul 3;13:10752. doi: 10.1038/s41598-023-37706-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of PRMT5 induces autophagy. (a) Immunoblot (IB) analysis of whole cell lysates (WCL) derived from BT-549 cells depleted of PRMT5 by two independent sgRNAs. (b) IB of WCL derived from BT-549 cells treated with GSK3326595 (GSK) at indicated doses for 3 days. (c, d) Representative images of GFP-LC3 puncta and cells with more than 10 puncta were counted in BT-549 (c) and Hs 578T (d) cells treated with DMSO or 1 μM GSK for 3 days. Scale bar, 10 μm. Data are shown as mean ± SD of n = 3 independent experiments with a total of 50 cells counted per experiment. P values were calculated by Student’s t test. (e, f) Representative images of GFP-LC3-RFP puncta in BT-549 cells depleted of PRMT5. Scale bar, 10 μm. Cells with more than 5 GFP-LC3 and RFP-LC3 puncta were counted as positive and data are shown as mean ± SD of n = 3 independent experiments with a total of 100 cells counted per experiment. P values were calculated by Student’s t test. (g) IB analysis of WCL derived from BT-549 cells depleted of PRMT5. Cells were treated with chloroquine 20 μM (CQ) for 0, 4, 8 h before harvesting. Similar results were obtained in n ≥ 3 independent experiments in (a, b, g).