Skip to main content
. 2023 Jul 3;6:138. doi: 10.1038/s42004-023-00937-y

Fig. 4. Quinolone production in dependence of fatty acid availability.

Fig. 4

a β-Hydroxydecanoic acid incorporation in 4(1H)-quinolone biosynthesis in P. aeruginosa. Production levels of 2′-OH-NQ (b) and 2′-OH-NQNO (c) in P. aeruginosa PAO1 after feeding β-hydroxydecanoic acid. Production levels of d 2′-OH-NQ and 2′-OH-NQ + 1 Da; e 2′-OH-NQNO and 2′-OH-NQNO + 1 Da measured in P. aeruginosa PAO1 in control sample (orange labels) and after feeding 100 µM deuterated β-hydroxydecanoic acid (3d) to bacterial cultures (green labels). Predicted m/z distribution for 2′-OH-NQ [M + H]+: 288.1959 (100.0%), 289.1992 (19.5%), 290.2026 (1.8%). Predicted m/z distribution for 2′-OH-NQNO [M + H]+: 304.1908 (100.0%), 305.1941 (19.5%), 306.1975 (1.8%). Mean values are given for biological triplicates with the error bars representing the population standard deviation.