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. 2023 Jun 20;11:1208843. doi: 10.3389/fchem.2023.1208843

TABLE 4.

ABTS assay of antioxidant activity of commercial formulations of local anesthetics in an aqueous medium.

Local anesthetic Concentration (mg/mL) Absorbance values ABTS˙+ scavenging effect (%)
After 30 min After 2 h After 30 min After 2 h
Lidocaine hydrochloride 20 0.0249 0.0172 90.13 93.04
Bupivacaine hydrochloride 5 0.1383 0.1048 45.16 57.62
Ropivacaine hydrochloride 7.5 0.1324 0.1048 47.50 57.62
Lidocaine hydrochloride buffered to 7.4 pH 10 0.00395 N/P 96.20 N/P
Negative control 0 0.2522 0.2473 N/A N/A

ABTS˙+ scavenging effect was calculated using Eq. 1. N/A–not applicable. N/P–not performed.