The mRNA and protein expression levels of hepatic (A) FXR and (B) CYP7A1. (C) Hepatic western blot images. (D) Ileal western blot images. (E) The protein expression levels of hepatic SHP. The mRNA and protein expression levels of ileal (F) FXR, (G) FGF15, (H) ZO-1, and (I) Occludin. Data were expressed as mean± SEM. N=8 per group when mRNA was measured. N=3 when protein expression was measured. *p<0.05, **p<0.01, ***p<0.001 vs. ND group. #p<0.05, ##p<0.01, ###p<0.001 vs. PBS group. FXR, farnesol X receptors; CYP7A1, cytochrome P450 family 7 subfamily A member-1; FGF15, fibroblast growth factor 15; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SHP, small heterodimer partner; ZO-1, tight junction protein-1.