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. 2023 May 11;299(6):104816. doi: 10.1016/j.jbc.2023.104816

Figure 2.

Figure 2

CRN02481 decreases insulin secretion from primary isolated islets. Secreted insulin from batch incubation of (A) WT or (B) Sur1−/− primary isolated pancreatic islets stimulated with denoted glucose concentrations and treated with either vehicle control or CRN02481 (500 nM). For WT, CRN02481 treatment is compared to vehicle control: 3 mM glucose (F(1, 42) = 8.57, p = 0.005); 10 mM glucose (F(1, 38) = 4.53, p = 0.04); 25 mM glucose (F(1, 46) = 4.68, p = 0.04). For Sur1−/−, CRN02481 is compared to vehicle control: 3 mM glucose (F(1, 29) = 25.16, p = 2.42 × 10−5); 10 mM glucose (F(1, 34) = 19.45, p = 0.002); 25 mM glucose (F(1, 38) = 1.09, p = 0.30). Secreted insulin from batch incubation of (C) WT or (D) Sur1−/− primary isolated islets stimulated with denoted physiological amino acid mixture (AAM) concentrations and treated with either vehicle control or CRN02481 (500 nM). For WT, CRN02481 treatment is compared to Vehicle control: 0 mM AAM (F(1, 38) = 1.39, p = 0.24); 2 mM AAM (F(1, 34) = 0.03, p = 0.86); 4 mM AAM (F(1, 38) = 0.30, p = 0.58); 10 mM AAM (F(1, 34) = 0.98, p = 0.33). For Sur1−/−, CRN02481 is compared to Vehicle control: 0 mM AAM (F(1, 38) = 7.16, p =0.01); 2 mM AAM (F(1,38) = 15.63, p = 0.0003); 4 mM AAM (F(1, 38) = 2.96, p = 0.09), 10 mM AAM (F(1, 38) = 0.63, p = 0.43). p-values calculated with a one-way ANOVA Test. (Statistically significant comparison to Vehicle control: ∗p ≤ 0.05; ∗∗p ≤ 0.01; ∗∗∗p ≤ 0.001). Data represent mean ± SD compared to vehicle control. n = 4 to 5. WT, wild type.