Expression of ankyrin-B and ankyrin-binding partners in the AnkBp.E1458G+/+heart lysates of aged mice.A and B, immunoblotting and quantitative analysis of AnkB normalized to GAPDH illustrating no changes in the AnkBp.E1458G+/+ hearts. C and D, immunoblotting and quantitative analysis of Na+/Ca2+ exchanger (NCX) normalized to GAPDH, showing no changes in the AnkBp.E1458G+/+ hearts. E and F, immunoblotting and quantitative analysis of Na+, K+-ATPase (NKA) normalized to GAPDH, illustrating a significant reduction in the AnkBp.E1458G+/+ hearts around 6 months of age. G, no significant changes in the expression of AnkG normalized to GAPDH in the AnkBp.E1458G+/+ hearts (con and AnkBp.E1458G+/+, N = 4). All data passed Shapiro–Wilk normality tests, and unpaired t-tests were performed. AnkB, ankyrin-B.