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. Author manuscript; available in PMC: 2023 Jul 4.
Published in final edited form as: Sci Transl Med. 2022 Aug 31;14(660):eabj7465. doi: 10.1126/scitranslmed.abj7465

Fig. 4. Activated neutrophils are transcriptionally primed for prothrombotic adhesion and migration.

Fig. 4.

(A) The volcano plot from RNA sequencing of peripheral blood neutrophils demonstrates more than 2000 differentially expressed genes between K2KO and LysM cells. (B) Gene Ontology (GO) biological process analysis of differentially expressed genes from K2KO neutrophil RNA sequencing is shown. % Gene set represents the proportion of the entire process that is affected by loss of KLF2. (C) The overlap of enriched pathways between K2KO neutrophils and neutrophils from humans with APS is shown. (D) Results of a transwell migration experiment using N-formyl-Met-Leu-Phe (fMLP) as chemoattractant for LysM and K2KO neutrophils are shown (n = 10 to 12). (E) Peritoneal lavage after fMLP intraperitoneal injection was used to quantify neutrophil migration in LysM (n = 7) and K2KO (n = 5) mice. (F and G) Ly6G-stained neutrophils were quantified along vessel walls early after (F) carotid artery injury (10 min) and (G) IVC ligation (4 hours) are shown for LysM and K2KO mice (n = 4 to 5). (H) Neutrophils were quantified along vessel walls early after carotid artery injury in IgG-injected (n = 5) or aPL-injected (n = 4) WT mice. *P < 0.05, **P < 0.01, and ***P < 0.001 by unpaired, two-tailed Student’s t test.