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. 2023 Jul 4;20:53. doi: 10.1186/s12987-023-00448-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The SHR choroid plexus demonstrates upregulation of pNKCC1. a Confocal microscopy images demonstrating apical localization of NKCC1 (red, top panel) and pNKCC1 (red, lower panel) in SHR choroid plexus. Cell nuclei are stained with DAPI (blue). Scale bar: 50 μm. b Representative immunofluorescence images of the choroid plexus from WKY rats (left panels) and SHRs (right panels) stained for NKCC1 (red, top panels), pNKCC1 (red, lower panels), and cell nuclei (DAPI, blue). Scale bar: 100 μm. c-d Quantification of NKCC1 (c) or pNKCC1 (d) fluorescence intensity in choroid plexus from WKY rats and SHRs, n = 6 in each group. e Western blot of pNKCC1 in choroid plexus from WKY rats and SHRs. β-actin served as loading control. f quantification of pNKCC1 abundance in choroid plexus from WKY rats (n = 4) and SHRs (n = 4) normalized to β-actin. Error bars represent standard deviation and statistical significance was tested with an unpaired two-tailed t-test or a Mann-Whitney test. * P < 0.05, **P < 0.01, NS = not significant