Fig. 3.

TCR promotes oxidative stress-dependent mutagenesis. (A) Median rifampicin concentration that allows for growth in the indicated strains after 6 d of evolution. 50 mM thiourea was included in the media when indicated. n = 23 (wt − thiourea), 12 (wt + thiourea), 34 (ΔuvrA − thiourea), 12 (ΔuvrA + thiourea), 24 (Δmfd − thiourea), 12 (Δmfd + thiourea) biological replicates. (B) Fold increase in the median rifampicin concentration that allows for growth in the indicated strains after 6 d of evolution in the presence or absence of 50 mM thiourea. Statistical significance was assessed with a two-tailed Mann–Whitney U test.