Fig. 1.

WBI substantially decreases the number of pathogen-specific circulating memory CD8+ T cells. (A) Experimental design: 10⁴ naive Thy1.1⁺ P14 CD8⁺ T cells were adoptively transferred into Thy1.2⁺ naïve hosts, followed by LCMV-Arm infection to generate memory P14 CD8 T cells. Forty-five days later, the memory P14 chimeric mice were either exposed to mock (0Gy) or 5Gy WBI and 1 d later, the mice were killed for further analysis. (B) The number of memory P14 CD8 T cells was determined in blood a day prior and after WBI and the number of memory P14 CD8 T cells was determined in the indicated organs 1 d after WBI (C) Frequency of CD62L⁺ P14 memory CD8 T cells was assessed in blood a day prior and after WBI and frequency of CD62L⁺ P14 memory CD8 T cells was assessed in the spleen 1 d after WBI. All data are representative of at least two independent experiments with 4 to 10 mice per group. *=P < 0.05, **=P < 0.01, ***=P < 0.001. Error bars represent SEM.