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. 2023 Jun 26;120(27):e2301067120. doi: 10.1073/pnas.2301067120

Fig. 6.

Fig. 6.

Cuboplexes show higher transfection efficiency of siRNA and mRNA compared to lipoplexes. (A) siRNA knockdown of GFP in HeLa–GFP cells with IL-LNPs, cuboplexes, and lipoplexes. GFP expression is determined by fluorescence intensity measured by flow cytometry (n = 3, data presented as mean ± SD). (B) Luciferase activity of HeLa cells transfected with firefly luciferase mRNA with mRNA alone, cuboplexes, and lipoplexes (n = 5, data presented as mean ± SD). (C) Luciferase activity of HeLa cells transfected with firefly luciferase mRNA using IL-LNPs and LNP–mRNA complexes with composition of GMO/DOPC:Chol:DOTAP:DOPE-PEG 55:30:14:1 mol% (n = 5, data presented as mean ± SD). Significance between groups was calculated with two-sided Student’s t-test and indicated as *P ≤ 0.05, **P ≤ 0.01, or ***P ≤ 0.001.