TABLE 1.

Major commercially available kits for TCR profiling.

	Milaboratories	NEBNext® immune sequencing	SMARTer TCR a/b profiling	iRepertoire
Species	Mouse, human, and monkey	Mouse and human	Mouse and human	Mouse and human
Protocol	Multiplex PCR	5′ RACE	SMART technology	arm-PCR
UMI	Yes	Yes	No	No
Input material	Up to 500 ng	10 ng–1 µg RNA or RNA- contained cells	10 ng–3 µg of RNA or 50–10,000 cells	50 ng–1 µg RNA
Sequencing	Illumina	Illumina Miseq®	Illumina Miseq®	Illumina
Analysis	MIXCR and MIGEC	Presto (Galaxy)	Any softwares	iRepertoire

SMART, Switching Mechanism at 5′ End of RNA template; arm-PCR, amplicon rescued multiplex PCR.